SVF MASTER GOLDEN STANDARD IN MECHANICAL METHOD
The world’s first product that can be compared to enzymatic products in terms of results, without containing adipose tissue.
Not Fat Injection: Cell Isolation
Highest Concentration
While many products claim to create SVF by making fat injectable, this system achieves cell-by-cell separation within all fat tissues, concentrating the full potential benefit into just 4ml.
What did they say?
Alper Murat Ulaşlı, MD, Prof.
An enzyme‑free technique enables the isolation of a large number of adipose‑derived stem cells at the bedside
“Adipose tissue-derived stromal cells (ADSCs) play a significant role in the research and applications of regenerative medicine due to their ability to be rapidly isolated in high quantities. Their purity, pluripotency, differentiation capacity, and stem cell marker expression can vary greatly depending on the technique and tools used for extraction. In my study published in Nature Scientific Reports in 2023, I investigated the performance of the widely used Microlyzer technology for adipose-derived stem cell isolation compared to the gold standard enzymatic method, as well as the advantages and cell quality.’’
Michele L. Zocchi, MD, Prof.
New perspectives in regenerative medicine and surgery: the bioactive composite therapies (BACTs)
“We analyzed the main causes of cell death and explored strategies for prevention. We present all the technical steps to achieve the most efficient regenerative effect in various clinical and pathological conditions, along with different mixing methods using their key components. In my Masterclass study published in the european journal of plastic surgery (ejps) in 2021, I shared the results that I obtained from different disciplines using new techniques.”
Pietro Gentile, MD, Prof.
Lipofilling Enriched with Adipose-Derived Mesenchymal Stem Cells Improves Soft Tissue Deformities and Reduces Scar Pigmentation: Clinical and Instrumental Evaluation in Plastic Surgery
’’This study aimed to evaluate the long-term effects of adipose tissue-derived mesenchymal stem cells combined with commonly used fat grafting in treating scar pigmentation changes and soft tissue deformities. In my study published in Aesthetic Plastic Surgery in 2023, I explained the impact of fat injections on scar treatments and the regenerative effects observed in the long term.’’
Michele L. Zocchi, MD, Prof.
Regulatory, ethical, and technical considerations on regenerative technologies and adipose-derived mesenchymal stem cells
‘’Since the publication of my groundbreaking study in 1988, which introduced the first Adipose-Derived Stromal Vascular Fraction (SVF) obtained through my specially developed and patented manual centrifuge system, many years have passed. Over the years, I have developed and tested various techniques that offer both mechanical and enzymatic approaches to SVF isolation, becoming an expert in the field. When evaluating a new medical device for SVF concentration, preparation, and isolation; I always consider ease of use, speed, efficacy, and cost. In my Masterclass study published in the European Journal of Plastic Surgery (EJPS) in 2019, I extensively discussed the Microlyzer SVF, which has surpassed other devices I have experienced in terms of meeting all these important criterias and delivering the most satisfying results.’’
Science at every stage
We extract the fat using the right equipment
Everything inside is single-use because safety comes first.
We purify it
Using special syringes, we compress the fat at high speeds to remove unwanted blood, water, and free triglycerides.
We release the cells
We pass them through blades one by one to separate the cells from the fat tissue and release them.
We isolate the SVF cells
We centrifuge the adipose tissue by dividing it into two tubes. Then, we pass the resulting pellet through a 41-micron filter to ensure the safety of the injection.
Visible results
The results are so high that it is possible to see the cells separated from the fat tissue as pellets at the bottom of the tube with the naked eye.
But there is no fat, no RBCs, and no leukocytes in it.
How do we know?
Here are the Flow Cytometry results:
CD73 (61.6%), CD90 (81.2%), and CD105 (2.3%) were measured, whereas CD34, CD29, CD14, CD11b, and HLA-DR were excluded to minimize possible counting errors
Fresh counting results with a fluorescence microscope
2.4 Million Nucleated Cells/ml, 92.8% Viability
8.9 Million Total Cells with High Regenerative Capacity in a 4ml Dose.